Read the following and answer any four questions from 9(i) to 9(v) given below:
Bioreactors are considered as vessels in which raw materials are biologically converted into specific products by microbes, plant and animal cells or their enzymes. They are used for large scale production as they provide optimum growth conditions such as temperature, pH, substrate, vitamins, oxygen and salts for obtaining desired product. Most commonly used bioreactors are of stirring type which include simple stirred tank bioreactor and sparged stirred-tank bioreactor.
- Bioreactor are useful in:
- Amplifying a gene.
- Isolation of genetic material.
- Processing large volume of culture.
- Infecting DNA in a cell.
- Which of the following is essential to obtain desired product in a bioreactor?
- Size of the bioreactor.
- Sterile condition.
- Quantity of the raw material.
- All of these.
- Assertion: The stirred-tank is well suited for large scale production of microorganisms under aseptic conditions.
Reason: In sparged stirred tank bioreactor, surface area for oxygen transfer is increased.
- Both assertion and reason are true and reason is the correct explanation of assertion.
- Both assertion and reason are true but reason is not the correct explanation of assertion.
- Assertion is true but reason is false.
- Both assertion and reason are false.
- Growth condition that could affect the quality of obtained product in a bioreactor are:
- Temperature and pH only.
- pH and oxygen supply only.
- Temperature and oxygen supply only.
- Temperature, pH and oxygen supply.
- Vessels in which raw materials are biologically converted into specific products are.
- Bioreactors.
- Fermentors.
- Gene guns.
- Both (a) and (b).