Question
DNA being hydrophilic, cannot pass through the cell membrane of a host cell. Explain how the recombinant DNA gets introduced into the host cellto transform the latter.
✓
Answer
Introduction of rDNA into host cell:
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Microinjection: In this method, the recombinant DNA is directly injected into the nucleus of an animal cell.
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Gene gun/ Biolistics: In this method, used for plant cells, the cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA.
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Heat shock method: In this method, the rDNA is forced into the competent cell by incubating the cell with rDNA on ice followed by placing them briefly at 42°C (heat shock) and then putting them back on Ice.
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