Question
Enumerate the steps which are involved in recombinant DNA technology.
✓
Answer
- Isolation of DNA.
- Fragmentation of DNA by restriction endonucleases.
- Isolation of desired DNA fragment.
- Ligation of DNA fragment into vector.
- Transferring recombinant DNA into host.
- Culturing host cells at large scale.
- Extraction of the desired product.
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