Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

PCR = Polymerase chain reaction (in vitro method) is a molecular biological technique for enzymatically replicating DNA without using a living organism, such as E. coli or yeast.

3 steps in PCR are-

1. Denaturation of desired double strand DNA-to ssDNA
2. Annealing of primer to ssDNA (single standard).
3. Extension of primer by Taq DNA polymerase isolated form Thermits aquaticus.

Uses- Amplification of desired gene/ gene cloning.

Advantage- More output, greater efficiency, less error prone, less human interference and cyclic and automated.