Explain briefly: PCR - Vidyadip

Question

Explain briefly:
PCR

✓

Answer

PCR = Polymerase chain reaction (in vitro method) is a molecular biological technique for enzymatically replicating DNA without using a living organism, such as E. coli or yeast.
3 steps in PCR are-

Denaturation of desired double strand DNA-to ssDNA
Annealing of primer to ssDNA (single standard).
Extension of primer by Taq DNA polymerase isolated form Thermits aquaticus.

Uses- Amplification of desired gene/ gene cloning.

Advantage- More output, greater efficiency, less error prone, less human interference and cyclic and automated.

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