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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes3 Marks
Question
  1. Explain how to find whether an E. coli bacterium has transformed or not, when a recombinant DNA bearing ampicillin-resistance gene is transferred into it.
  2. What does the ampicillin-resistant gene act as, in the above case?
✓

Answer

  1. The recombinant/ transformaot can be selected out from the non-recombinants/ non-transformants by Plating the transformants on ampicilin-containing medium. The transformants will grow in it, while the non-transformants will not grow.
  2. It acts as a selectable marker.

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