Biotechnology and its Applications — BIOLOGY STD 12 Science — Question

Describe: Genetic engineered insulin

Read the following text carefully and answer the questions that follow:
A pathogen is defined as an organism causing disease to its host, with the severity of the disease symptoms referred to as virulence. Pathogens are taxonomically widely diverse and comprise viruses and bacteria as well as unicellular and multicellular eukaryotes. The immune system of a person is suppressed. He was found positive for a pathogen in the diagnostic test ELISA.

i. Name the disease, the patient is suffering from. (1)
ii. Which pathogen is identified by ELISA test? (1)
iii. Which cells of the body are attacked by the pathogen? (2)
OR
Suggest preventive measures of the infection. (2)

Read the following and answer any four questions from 7(i) to 7(v) given below:

The vectors are DNA molecules that can carry a foreign DNA segment and replicate inside the host cell. Vectors may be plasmids, bacteriophages (viruses that attack bacteria), cosmids, yeast artificial chromosomes (YACs), Bacterial artificial chromosomes (BACs) and viruses. The most widely used, versatile, easily manipulated vector pBR 322 is an ideal plasmid vector. Features that are required to facilitate cloning into a vector includes origin of replication (Ori) which is a specific sequence of DNA bases responsible for initiating replication, selectable marker genes and cloning sites

1. Pin pBR 322 denotes that it is a:

1. plasmid
2. prokaryote
3. protist
4. plant cell.

2. Ori is a specific DNA sequence that help in.

1. Attachment of primers.
2. Initiation of replication.
3. Extension of DNA base.
4. Initiation of denaturation.

3. A and B shown in the figure respectively indicates:

1. Pvu II and Cla I.
2. ROP and Sal I.
3. amp^R and tet^R.
4. tet^R and amp^R.

4. Selectable markers in vector,

1. Are responsible for replication.
2. Help in selecting transformants from non-transform ants.
3. Code for proteins involved in the replicating plasmids.
4. Contain unique recognition sites.

5.  Plasmid vectors are:

1. dsDNA molecule
2. Extra-chromosomal
3. Present in bacteria and yeast
4. All of these.

Microorganisms include bacteria, viruses, fungi and protozoa. In our mind, we presume, most of the time, that microbes are always harmful. Microbes are, of course, the causal agents of many infections diseases of plants and animals including humans but they also have lots of beneficial role. Lactic acid bacteria (LAB) are one of this kind of useful group. These are Gram positive, non-sporulating, either rod-shaped or spherical bacteria. They produce lactic acid in milk products as major metabolic end product of carbohydrate fermentation. LAB are considered as natural fermentors. Lactobacillus is a common LAB which converts lactose sugar of milk into lactic acid, that causes coagulation and partial digestion of milk protein casein. Milk is then changed into curd, yoghurt and cheese. Lactobacillus is also used in probiotics which have potentially beneficial effect on gut ecosystem of humans. Some other probiotic strain used belong to the Genus Bifidobacterium.

1. Which of the following is not considered as microorganisms?

1. Bacteriophage.
2. Streptococcus.
3. Porphyra.
4. Staphylococcus.

2. Select the incorrect option regarding the characteristics of lactic acid bacteria.

1. They are rod-shaped or spherical.
2. They are Gram positive.
3. They take part in carbohydrate fermentation.
4. They are acid intolerant.

3. Which of the following is not a lactic acid producing bacteria?

1. Streptococcus.
2. Lactococcus.
3. Saccharomyces.
4. Enterococcus.

4. Probiotics are:

1. Gut friendly live bacteria.
2. Acid balancing alternated bacteria.
3. Beneficial amount of dead bacteria.
4. Gram negative attenuated bacteria.

5. Assertion: Lactobacillus bacteria do not retain crystal violet stain while staining.

Reason: Lactobacillus have a very thin layer of peptidoglycan layer in their cell wall.

1. Both assertion and reason are true and reason is the correct explanation of assertion.
2. Both assertion and reason are true but reason is not the correct explanation of assertion.
3. Assertion is true but reason is false.
4. Both assertion and reason are false.

Read the following and answer any four questions from (i) to (v) given below:

The anther is a four-sided structure consisting of four microsporangia located at the corners, two in each lobe. TI1e microsporangia develop further and become pollen sacs. In a transverse section, a typical microsporangium appears near circular in outline. It is generally surrounded by four wall layers-the epidermis, endothecium, middle layers and the tapetum.

1. A dithecous another consists of (A) microsporangia, (B) in each lobe.

Select the option that correctly fills the blanks.

S. No	A	B
(a)	Four	Two
(b)	Two	One
(c)	Two	Two
(d)	Four	One

2. The given diagram shows microsporangium of a mature anther. Identify A, B and C.

(a)	A-Middle layer	B-Endothecium	C-Tapetum
(b)	A-Endothecium	B-Tapetum	C- Middle layer
(c)	A-Endothecium	B-Middle layer	C-Tapetum
(d)	A-Tapetum	B-Middle layer	C-Endothecium

3. The function of labelled part X is:

1. Dehiscence.
2. Mechanical.
3. Nutrition.
4. Protection.

4. Select the incorrect statement.

1. Microsporangium is generally surrounded by four wall layers-epidermis, endothecium, middle layers and tapetum.
2. Outer three layers perform functions of protection and dehiscence of anthers.
3. Cells of tapetum possess dense cytoplasm and generally have more than one nucleus.
4. Cells of tapetum undergo meiosis and produce microspore tetrads.

5. Which function oftapetum is correct?

1. Helps in pollen wall formation.
2. Transportation of nutrients to inner side of another.
3. Synthesis of callase enzyme for separation of microspore tetrads.
4. All of these.

Explain with diagram - Hershey and Chase experiment.###On the basis of experimental proof explain the diagram that DNA is genetic material.###How did Hershey and Chase differentiate between DNA and protein in their experiment while proving that DNA is the genetic material?

Give a detailed description of human male reproductive system. (Diagram required)

Read the following and answer any four questions from (i) to (v) given below:
Cross pollination is the transfer of pollen grains from the anther of a one flower to the stigma of a genetically different flower. It is performed with the help of an external agency which may be abiotic (e.g., wind, water) or biotic (e.g., insects, birds, bats, snails). The diagram shows the carpel of an insect pollinated flower.

1. What is the most likely reason for non germination of pollen grain Z?

1. Pollen grains X and Y were brought to the stigma earlier, therefore, their germination inhibited the germination of pollen grain Z.
2. Pollen grain Z was brought to the flower by wind, while pollen grains X and Y were brought to the flower by insect.
3. Pollen grain Z lacks protrusions that allow it to adhere properly onto the stigma surface.
4. Pollen grain Z comes from a flower of an incompatible species.

2. Which of the following best describes the function of the pollen tube?

1. It acts as a conduit to transport male gametes from the anther to the ovule.
2. It acts as a conduit to transport male gametes from the stigma to the ovule.
3. It contains key nutrients that serve to nourish the newly-formed zygote.
4. It digests the tissues of the stigma, style, and ovary.

3. Pollination of a flower in which the pollen is carried by an insect is called:

1. Anemophily.
2. Ornithophily.
3. Entomophily.
4. Malacophil.

4. Refer to the given characteristics of some flowers.

1. The stamens hang out of the flower, exposing the anthers to the wind.
2. The pollen grains are tiny and light.
3. The flower has a sweet scent.
4. The flower petals are brightly colored.

5. Pollenkitt is generally found in:

1. Anemophilous flowers.
2. Entomophilous flowers.
3. Ornithophilous flowers.
4. Malacophilous flowers.