Question
How and why is the bacterium Thermus aquaticus employed in DNA technology? Explain.

Or

Name the source of the DNA polymerase used in PCR technique. Mention why it is used?

Or

Give the name of the organism from where the thermostable DNA polymerase is isolated. State its role in genetic engineering.

Answer

Bacterium Thermus aquaticus is a source of enzyme tag polymerase, As it is a thermostable enzyme and work at high temperature, it is used to amplify DNA in vitro by PCR. The amplified fragment of desired DNA can be used to ligate with the vector for further cloning

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