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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes3 Marks
Question


  1. Identify the selectable markers in the diagram of E. coli vector shown above.
  2. How is the coding sequence of a-galactosidase considered a better marker than the ones identified by you in the diagram? Explain.
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Answer

  1. a = ampicillin, b = EcoR-I, c = Hind-III, d = tetracycline.
  2. Insertion of alien gene into coding sequence of a-galactosidase, results into inactivation of the enzyme// (insertion inactivation), these colonies do not produce any colour (in the presence of chromogenic substrate) hence are identified as recombinant colonies.

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