Question
Microbes are useful in household products. Explain this with suitable examples

Answer

We use microbes. Our products are derived from them everyday.
(1) Curd: LABs are lactic acid bacteria.
→ They grow in milk and convert it to curd.
→ During growth, the LAB produce acids that coagulate and partially digest the milk proteins.
→ A small amount of a curd added to the fresh milk as inoculum or starter contain millions of LAB, which at suitable temperatures multiply, thus converting milk to curd.
→ LAB improves milk quality by increasing vitamin B12.
→ In our stomach too, the LAB play very beneficial role in checking diseases causing microbes.
2. Cheese:
→ Cheese is an edible substance. Microbes are used at different stages of cheese. making.
→ Different varieties of cheese are known by their characteristic texture, flavour and taste which is given by specific microbes used in the cheese production.
→ The large holes in "swiss cheese" are due to production of a large amount of carbon dioxide by a bacterium named Propionibacterium sharmanii.
→ The "Roquefort cheese" are ripened by growing a specific fungi on them, which gives them a particular flavour.
3. Batter:
→ The batter of dosa and idli is fermented by using bacteria. This batter looks puffed because of the production of carbon dioxide.
→ The dough, which is used for making bread, is fermented using saccharomyses cerevisiae- baker's yeast.
4. Toddy:
→ Some traditional drink and food is also produced by the help of microbes through fermentation.
→ In South India, traditional drink toddy is made by fermenting sap from palms.
5. Other food items:
→ To produce different food items fish, soyabean, bamboo etc. are passed through fermentation.

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Read the following and answer any four questions from (i) to (v) given below:

Gene manipulation is a fast emerging science. It started with development of recombinant DNA molecule. It is named variously as DNA manipulation biotechnology, recombinant DNA technology and genetic engineering. This technology, that mostly involves cutting and pasting of desired DNA fragments, is based on two important discoveries in bacteria, i.e., presence of plasmid in bacteria and restriction endonucleases. Paul Berg was able to introduce a gene ofSV-40 into a bacterium. The science of recombinant DNA technology took birth when Cohen and Boyer (1973) were able to introduce a piece of gene containing foreign DNA into plasmid of E.coli.

  1. Biotechnology is also known as:
  1. DNA manipulation biotechnology.
  2. Recombinant DNA technology.
  3. Genetic engineering.
  4. All of these.
  1. A bacterial plasmid is a/ an,
  1. Extra chromosomal material that do not replicate.
  2. Extra chromosomal material that undergo replication with or without chromosomal DNA.
  3. Tubular structures that help in conjugation.
  4. Bristle like solid structure that help in adhesion.
  1.  Father of genetic engineering is:
  1. Paul Berg.
  2. Arber.
  3. Nathan.
  4. Smith.
  1. Which of the following is used by Paul Berg to introduce a gene of SV-40 in a bacterium?
  1. E. coli.
  2. cos-plasmids.
  3. Lambda phage.
  4. None of these.
  1.  Read the given statements and select the correct option.

Assertion: Biotechnology started with the development of recombinant DNA molecule.

Reason: Biotechnology mostly involves cutting and pasting of desired DNA fragments.

  1. Both assertion and reason are true and reason is the correct explanation of assertion.
  2. Both assertion and reason are true but reason is not the correct explanation of assertion.
  3. Assertion is true but reason is false.
  4. Both assertion and reason are false.
What is chromosomal disorder? Explain any two aneuploidy with characters in humans.###Mention any two autosomal genetic disorders with their symptoms.
Read the following and answer any four questions from (i) to (v) given below:

The process of translation requires transfer of genetic information from a polymer of nucleotides to synthesise a polymer of amino acids. The relationship between the sequence of amino acids in a polypeptide and nucleotide sequence of DNA or mRNA is called genetic code. George Gamow suggested that in order to code for all the 20 amino acids, code should be made up of three nucleotides.

  1. What is a codon?
  1. A length of DNA which codes for a particular protein.
  2. A part of the tRNA molecule to which a specific amino acid is attached.
  3. A part of the tRNA molecule which recognises the triplet code on the messenger RNA.
  4. A part of the messenger RNA molecule that has a sequence of bases coding for an amino acid.
  1. Three consecutive bases in the DNA molecule provide the code for each amino acid in a protein molecule. What is the maximum number of different triplets that could occurs?
  1. 16
  2. 20
  3. 24
  4. 64
  1. Listed below are some amino acids and their corresponding mRNA triplets.
Amino acid
mRNA triplet
Phenylalanine
UUU
Lysine
AAG
Arginine
CGA
Alanine
GCA
Which DNA sequence would be needed to produce the following polypeptide sequence? Alanine-Arginine-Lysine-Phenylalanine

  1. $\text{CGT}\ \ \ \text{GCT}\ \ \ \text{TTC}\ \ \ \text{AAA}$

  2. $\text{CGT}\ \ \ \text{GCT}\ \ \ \text{TTC}\ \ \ \text{TTT}$

  3. $\text{CGU}\ \ \ \text{GCU}\ \ \ \text{UUC}\ \ \ \text{AAA}$

  4. $\text{CGU}\ \ \ \text{GCU}\ \ \ \text{UUC}\ \ \ \text{TTT}$

  1. Identify the non-sense codons among the following.
  1. AUG
  2. GUG
  3. UAA
  4. UGG
  1. A polypeptide is made using synthetic mRNA molecules as shown.
Synthetic mRNA used
Polypeptide produced
UUUAAAUUUAAA
Phenylalanine-Iysine-phenylalanine-Iysine

What are the DNA codes for the amino acids phenylalanine and lysine?

S.no
Phenylalanine
Lysine
(a)
AAA
TTT
(b)
AAA
UUU
(c)
GGG
CCC
(d)
TTT
GGG
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Restriction endonuclease was isolated for the first ti me by W. Arb er in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called as molecular scissors or biological scissors. Three types of restriction endonucleases are Type I, Type II and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC in DNA duplex and cut strands between G and A.

  1. Only type II restriction enzymes are used in gene manipulation because
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  2. It consists of three different subunits.
  3. It makes cleavage or cut in both the strands of DNA molecule.
  4. Both (a) and (c).
  1. Which of the following ions are used by restriction endonucleases for restriction?
  1. Mg2+ ions
  2. Mn2+ ions
  3. Na2+ ions
  4. K2+ ions
  1.  Restriction endonuclease was isolated for the first time in a:
  1. Plant cell.
  2. Animal cell.
  3. Prokaryotic cell.
  4. Genninal cell.
  1. Restriction endonucleases are also called as molecular or biological scissors because,
  1. They cleave base pairs of DNA only at their terminal ends.
  2. They cleave one or both the strands of DNA.
  3. They act only on single stranded DNA.
  4. None of these.
  1. Select the option that correctly states the working action of restriction endonuclease EcoR I on DNA sequence GAATTC
  1. $\ \ \ \ \ \ \ \ \ \ \ \ \downarrow\\5'-\text{GAATTC}-3'\\3'-\text{CTTAAG}-5'\\\ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \uparrow$

  2. $ \ \ \ \ \ \ \ \ \ \ \downarrow\\5'-\text{GAATTC}-3'\\3'-\text{CTTAAG}-5'\\\ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \uparrow$

  3. $\ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \downarrow\\5'-\text{GAATTC}-3'\\3'-\text{CTTAAG}-5'\\\ \ \ \ \ \ \ \ \ \uparrow$

  4. $\ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \downarrow\\5'-\text{GAATTC}-3'\\3'-\text{CTTAAG}-5'\\\ \ \ \ \ \ \ \ \ \ \ \ \ \ \uparrow$

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S. No Column I S. No Column II
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(ii) Cotton bollworm 2. Biopiracy.
(iii) Eli Lilly 3. Emphysema.
(iv) Basmati Rice 4. ADA deficiency.
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In a study to test a new vaccine against a viral disease, mouse model testing is done. In this process, mice are vaccinated and their blood samples were tested. Mice developed mild disease symptom. After few days those mice were again infected with the virus. This time they do not show any disease symptoms. Their blood samples were tested. Two graphs show antibody concentration for the first and second infection in mice blood.

Based on the above information, answer the following questions.

  1. P and Qin the given graphs indicate.
  1. IgM and IgG respectively.
  2. IgG and IgM respectively
  3. IgG and IgM respectively
  4. IgM and IgA respectively.
  1. Which form of pathogen is used in vaccination?
  1. Activated and strong pathogenic antigen.
  2. Inactivated and weakened pathogenic antigen.
  3. Hyperactive and strong pathogen.
  4. Preformed antibodies.
  1. Which of the following is incorrect for P?
  1. It is the most abundant class of lg.
  2. It is found in blood, lymph, and intestine.
  3. It is unable to cross the placental barrier.
  4. It is a monomer.
  1. How does vaccination work?
  1. The immune system produces antibodies which stay in the blood.
  2. Memory lymphocytes remain in the body to fight off any future infection with the same pathogen.
  3. The dead pathogen stays in the body and constantly stimulates the immune system.
  4. All of these.
  1. Assertion: Mice do not show any disease symptoms during second exposure to the pathogenic virus.

Reason: The antibody production is accelerated and more intense during secondary immune response.

  1. Both assertion and reason are true, and reason is the correct explanation of assertion.
  2. Both assertion and reason are true, but reason is not the correct explanation of assertion.
  3. Assertion is true, but reason is false.
  4. Both assertion and reason are false.
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