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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes2 Marks
Question
Study the diagram given below and answer the following questions:

  1. Why have DNA fragments in band 'D' moved farther away in comparison to those in band 'C'?
  2. Identify the anode end in the diagram.
  3. How are these DNA fragments visualised?
✓

Answer

  1. DNA fragments in band D are smaller than those of band C.
  • The DNA fragments separate according to their size through the sieving effect provided by the gel; hence the smaller fragments move farther away than the larger ones.
  1. End towards B.
  2. The gel containing DNA fragments is stained with ethidium bromide and exposed to UV radiation; orange-coloured bands (of DNA) become visible.

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