MCQ
The whole cycle of gene amplification requires:
- A15 minutes
- B30 minutes
- C3 hours
- D24 hours.
Explanation:
The denaturation stage of PCR takes about 1–2 minutes. The thermocycler then lowers the temperature to about 50° to 60°C, which allows the short, oligonucleotide primers to anneal to their complementary sequences on the single-stranded DNA molecules. The annealing stage of PCR lasts about 30 seconds. Hence, the whole cycle of gene amplification requires 30 minutes.
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