Question
What is recombinant DNA? For creating recombinant vector molecule, can two different restriction enzymes be used for cutting vector and source DNA?
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Answer
When a specific gene sequence is linked with plasmid vector with the help of DNA ligase, a new combination of circular autonomously replicating DNA is formed and it is called recombinant DNA.
No, recombinant vector can be created only if the same restriction enzyme cuts both the vector DNA and the source DNA, as their palindromic sequences should be same.
No, recombinant vector can be created only if the same restriction enzyme cuts both the vector DNA and the source DNA, as their palindromic sequences should be same.
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