Biotechnology — BIOLOGY STD 12 Science — Question

1. Palindrome is a sequence which when read on opposite strands of DNA (3′ to 5’ or 5’ to 3’), reads same.
2. When the enzyme EcoRI recognizes this sequence, it breaks each between the A and G residues.
3. In palindrome, the base sequence of second half in DNA represents the mirror image of the base sequence of the first half.
4. Palindromes are actually groups of letters which form the same word when read in both forward and backward directions.

For example, recognition sequence of by the enzyme EcoRI is a palindrome.
3′ —— – C T T A A G—–5′
5′ —— – G A A T T C—–3′

Same restriction enzyme must be used to cut vector and donor DNA, because it will produce fragments with the same complementary sticky ends, making it bond formation possible between them.