$\text{f}_0=\frac{1}{20\text{D}}=0.05\text{m}=5\text{cm},$ $\text{f}_\text{e}=\frac{1}{10\text{D}}=0.1\text{m}=10\text{cm.}$
D = 25cm, separation between objective & eyepiece= 20cm
For the minimum separation between two points which can be distinguished by eye using the microscope, the magnifying power should be maximum.
For the eyepiece, v0 = -25cm, fe = 10cm
So,
$\frac{1}{\text{u}_\text{e}}=\frac{1}{\text{v}_\text{e}}-\frac{1}{\text{f}_\text{e}}=\frac{1}{-25}-\frac{1}{10}$$=-\Big[\frac{2+5}{50}\Big]\Rightarrow\text{u}_\text{e}=-\frac{50}{7}\text{cm}$
So, the image distance for the objective lens should be,
$\text{v}_0=20-\frac{50}{7}=\frac{90}{7}\text{cm}$
Now, for the objective lens,
$\frac{1}{\text{u}_0}=\frac{1}{\text{v}_0}-\frac{1}{\text{f}_0}=\frac{7}{90}-\frac{1}{5}=-\frac{11}{90}$
$\Rightarrow\text{u}_\text{o}=-\frac{90}{11}\text{cm}$
So, the maximum magnifying power is given by,
$\text{m}=\frac{-\text{V}_0}{\text{u}_0}\Big[1+\frac{\text{D}}{\text{f}_\text{e}}\Big]$
$=\frac{\big(\frac{90}{7}\big)}{\big(-\frac{90}{11}\big)}\Big[1+\frac{25}{10}\Big]$
$=\frac{11}{7}\times3.5=5.5$
Thus, minimum separation eye can distinguish
$=\frac{0.22}{5.5}\text{mm}=0.04\text{mm}$






