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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes4 Marks
Question
Answer the following questions:
Explain how recombinants and non-recombinants are differentiated on the basis of colour production in the presence of a chromogenic substrate. Name that procedure.
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Answer

The procedure is called insertional inactivation.
In this method recombinants and non-recombinants are differentiated on the basis of the ability to produce colour in the presence of a chromosomic substrate. In this method, a rDNA is inserted in an enzyme- $\beta-$galactosidase which leads to inactivation of the enzyme which does not produce colour due to insertion.

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