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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes4 Marks
Question
  1. Describe the characteristics a cloning vector must possess.
  2. Why DNA cannot pass through the cell membrane? Explain. How is a bacterial cell made ‘competent’ to take up recombinant DNA from the medium?
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Answer

  1.  
  • Should have ori/origin of replication,
  • Has selectable marker, genes encoding for an antibiotic resistance/genes encoding for a - galactosidase,
  • Has cloningsite/recognition site, for the restriction enzyme to recognise.
  1. DNA is a hydrophilic molecule

Bacterial cell is made competent by treating with specific concentration of Ca++ ion/divalent ions, incubating the mon ice, heat shock for a short period and placing it back on ice again.

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