Download App

Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Gujarat BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes5 Marks
Question
  1. Describe the characteristics a cloning vector must possess.
  2. Why DNA cannot pass through the cell membrane? Explain. How is a bacterial cell made ‘competent’ to take up recombinant DNA from the medium?
✓

Answer

  1.  
  • Should have ori/origin of replication,
  • Has selectable marker, genes encoding for an antibiotic resistance/genes encoding for a - galactosidase,
  • Has cloningsite/recognition site, for the restriction enzyme to recognise.
  1. DNA is a hydrophilic molecule
Bacterial cell is made competent by treating with specific concentration of Ca++ ion/divalent ions, incubating the mon ice, heat shock for a short period and placing it back on ice again.

Need a full question paper?

Generate a complete, print-ready paper with questions like this in minutes — across 16+ boards, with answer keys.

Start Generating Free

Explore more

More "5 Marks Questions" from Biotechnology: Principles and Processes→Biotechnology: Principles and Processes — all question types→BIOLOGY STD 12 Science question papers→Gujarat Board STD 12 Science question papers→Gujarat Board question paper generator→

Similar questions

  1. How did Hardy-Weinberg explain that allelic frequencies in a population are stable and are constant from generation to generation?
  2. Why does genetic equilibrium get disturbed in a population? Give reasons.
→
Define an operon. Giving an example, explain an Inducible operon.
→
List some adverse effects of particulate matter on human health.
→
Explain the law of dominance by Punnet Square method.
→
List the various abiotic environmental factors.
→
Discuss the various types of positive interactions between species.
→
Write an article on vaccination and immunization.
→
Looking at the deteriorating air quality because of air pollution in many cities of the country, the citizens are very much worried and concerned about their health. The doctors have declared health emergency in the cities where the air quality is very severely poor.
  1. Mention any two major causes of air pollution.
  2. Write any two harmful effects of air pollution to plants and humans.
  3. As a captain of your school Eco-club, suggest any two programmes you would plan to organise in the school so as to bring awareness among the students on how to check air pollution in and around the school.
→
When does oogenesis start in humans? Name the three hormones and their source, linked with oogenesis. How do they influence the process of oogenesis? Explain.
→
Explain the development of a secondary oocyte (ovum) in human female from the embryonic stage upto its ovulation. Name the hormones involved in this process.
→