Question
Explain with diagram - Hershey and Chase experiment.###On the basis of experimental proof explain the diagram that DNA is genetic material.###How did Hershey and Chase differentiate between DNA and protein in their experiment while proving that DNA is the genetic material?

Answer

→ The unequivocal proof that DNA is the genetic material came from the experiments of Alfred Hershey and Martha Chase (1952). They worked with viruses that infect bacteria called bacteriophages.
→ The bacteriophage attaches to the bacteria and its genetic material then enters the bacterial cell.
→ The bacterial cell treats the viral genetic material as if it was its own and subsequently manufactures more virus particles.
→ Hershey and Chase worked to discover whether it was protein or DNA from the viruses that entered the bacteria.
→ They grew some viruses on a medium that contained radioactive phosphorus and some others on medium that contained radioactive sulfur.
→ Viruses grown in the presence of radioactive phosphorus contained radioactive DNA but not radioactive protein because DNA contains phosphorus but protein does not.
→ Similarly, viruses grown on radioactive sulfur contained radioactive protein but not radioactive DNA because DNA does not contain sulfur.
→ Radioactive phages were allowed to attach to E.coli bacteria.
→ Then, as the infection proceeded, the viral coats were removed from the bacteria by agitating them in a blender.
→ The virus particles were separated from the bacteria by spinning them in a centrifuge.
→ Bacteria which was infected with viruses that had radioactive DNA were radioactive, indicating that DNA was the material that passed from the virus to the bacteria.
→ Bacteria that were infected with viruses that had radioactive proteins were not radioactive.
→ This indicates that proteins did not enter the bacteria from the viruses.
→ DNA is therefore the genetic material that is passed from virus to bacteria.
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​​​​Read the following and answer any four questions from (i) to (v) given below: Edward Wilson described diversity at all levels of biological organisation ranging from macromolecules inside the cells to biomes. It is of three inter-related hierarchial levels-genetic diversity, species diversity and conununity ecosystem diversity. Species diversity is the variety in the number and richness of the species of a region. For example, the Western ghats have a greater amphibian species diversity than the Eastern ghats.
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  1. Species evenness.
  2. Species richness.
  3. Species equitability.
  4. Both (a) and (c).
  1. The table below gives the population (in thousands) of ten species (A - J) in four areas (I - IV) consisting of the number of habitats given within brackets against each. Study the table and answer the question which follows:
Area and number of habitats
Species and their population (in thousands) in the area
A
B
C
D
E
F
G
H
I
J
I(11)
23
12
0.52
6.0
-
3.1
1.1
9.0
-
10.3
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10.2
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0.62
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1.5
3.0
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1.1
11.2
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11.3
0.9
0.48
2.4
1.4
4.2
0.8
8.4
2.2
4.1
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3.2
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1.1
4.8
0.4
3.3
0.8
7.3
1.3
2.1
Which are out of I to IV shows maximum species diversity?
  1. II
  2. III
  3. IV
  4. I
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Population
Species
Species
Individuals
Population A
I
Mammals
3
II
Birds
2
III
Amphibians
2
Population B
I
Mammals
2
II
Mammals
2
III
Amphibians
1
Population C
I
Mammals
3
II
Mammals
2
III
Mammals
4
 
Maximum diversity
Minimum diversity
(a)
Population B
Population C
(b)
Population A
Population C
(c)
Population A
Population B
(d)
Population B
Population A
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A
83
F
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B
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G
20
C
9
H
18
D
2
I
23
E
1
J
15
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Species Richness
Evenness
(a)
Higher
Higher
(b)
Higher
Lower
(c)
Same
Lower
(d)
Same
Higher
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S. No
A
B
(a)
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Two
(b)
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One
(c)
Two
Two
(d)
Four
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(c)
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C-Tapetum
(d)
A-Tapetum
B-Middle layer
C-Endothecium
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