Question
How are the following used in biotechnology?
- Plasmid DNA
- Recognition sequence
- Gel electrophoresis
✓
Answer
- Plasmid DNA: It is often used for constructing recombinant DNA, by ligating the gene of interest with it; it is used as the cloning vector.
- Recognition sequences: These are the sequences of base pairs in DNA, which a restriction enzyme recognises and cuts the DNA
- Gel electrophoresis: It is used to separate the DNA fragments, which separate/ resolve according to their size through the sieving effect of the gel; the DNA is isolated from the gel by the process of elution.
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