Question
Which methodology is used while sequencing the total DNA from a cell? Explain it in detail.
✓
Answer
Methodology used:
- Sequence Annotation-total DNA from a cell is isolated, converted into random fragments of relatively smaller sizes, and cloned in suitable host using specialized vectors.
- The cloning resulted into amplification of each piece of DNA fragment.
- The fragments were sequenced using automated DNA sequencers, these sequences are then arranged based on some overlapping regions (present in them).
- This requires generation of overlapping fragments (for sequencing).
- Specialised computer based programmes were developed, and these sequences were subsequently annotated and assigned to each chromosome.
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