Biotechnology and its Applications — BIOLOGY STD 12 Science — Question

Read the following and answer any four questions from (i) to (v) given below:
DNA fingerprinting is a technique of determining nucleotide sequences of certain areas of DNA which are unique to each individual. Each person has a unique DNA fingerprint. Each fingerprint is the same for every cell, tissue and organ of a person. DNA fingerprinting is the basis of paternity testing in case of disputes.

1. The technique developed to identify a person with the help of DNA restriction analysis is known as.

1. DNA profiting
2. DNA fingerprinting
3. RFLP
4. Both (a) and (b).

2. For DNA fingerprinting, DNA is obtained from.

1. Blood
2. Hair root cells
3. Semen
4. All of these

3. During DNA fingerprinting, the radioactive probes.

1. Hybridise with DNA sample to form double stranded structure
2. Egrade the DNA
3. Create positive charge on DNA
4. Cut the DNA sample at various sites.

4. In India, DNA fingerprinting technique was developed by?

1. Dr. Lalji Singh
2. Alec Jeffreys
3. Dr. Khorana
4. None of these.

5. Which of the following is true about DNA fingerprinting?

1. VNTR is used as probe.
2. DNA samples are loaded on agarose gel electrophoresis.
3. It is based on identification of nucleotide sequence present on the DNA molecule.
4. All of these

Read the following and answer any four questions from (i) to (v) given below:
Gene manipulation is a fast emerging science. It started with development of recombinant DNA molecule. It is named variously as DNA manipulation biotechnology, recombinant DNA technology and genetic engineering. This technology, that mostly involves cutting and pasting of desired DNA fragments, is based on two important discoveries in bacteria, i.e., presence of plasmid in bacteria and restriction endonucleases. Paul Berg was able to introduce a gene ofSV-40 into a bacterium. The science of recombinant DNA technology took birth when Cohen and Boyer (1973) were able to introduce a piece of gene containing foreign DNA into plasmid of E.coli.

1. Biotechnology is also known as:

1. DNA manipulation biotechnology.
2. Recombinant DNA technology.
3. Genetic engineering.
4. All of these.

2. A bacterial plasmid is a/ an,

1. Extra chromosomal material that do not replicate.
2. Extra chromosomal material that undergo replication with or without chromosomal DNA.
3. Tubular structures that help in conjugation.
4. Bristle like solid structure that help in adhesion.

3. Father of genetic engineering is:

1. Paul Berg.
2. Arber.
3. Nathan.
4. Smith.

4. Which of the following is used by Paul Berg to introduce a gene of SV-40 in a bacterium?

1. E. coli.
2. cos-plasmids.
3. Lambda phage.
4. None of these.

5. Read the given statements and select the correct option.

Assertion: Biotechnology started with the development of recombinant DNA molecule.

Reason: Biotechnology mostly involves cutting and pasting of desired DNA fragments.

1. Both assertion and reason are true and reason is the correct explanation of assertion.
2. Both assertion and reason are true but reason is not the correct explanation of assertion.
3. Assertion is true but reason is false.
4. Both assertion and reason are false.

Distinguish between: Long day plants and Short day plants

Read the following and answer any four questions from (i) to (v) given below:
Regular change in temperature that occurs at specific intervals of time is called thennoperiodicity. It is of two types-diurnal and seasonal thermoperiodicity. Diumal periodicity refers to temperatures of day and night. It determines periods ofannual activity. In season periodicity different temperature prevails in different seasons of the year. 'Tiley favour different aspects of plant and animal life termed as phenology. For example in wheat, leaf growth requires a temperature of 10º-25ºC. Apple requires temperature below 7ºC for a period of 800 hrs before flowering and fruiting can occur. Low temperature is required for germination of some seeds as well as flowering in some plants. It also determines growth, reproduction, colour and morphology of animals. Both low and high temperature cause stress in organisms which is overcome by particular adaptations.

1. Some plants require low temperature treatment for flowering. This phenomenon is known as:

1. Photoperiodism.
2. Vemalisation.
3. Thermoperiodism.
4. None of these.

2. Animals found in arctic zones are called.

1. microtherms
2. Megatherms
3. Mesotherms
4. Hekistotherms

3. Which of the following parts of wheat plant grows maximum in temperature around 10º-25ºC?

1. Root
2. Seeds
3. Leaf
4. Stem

4. "Different temperatures prevail in different seasons of the year." It represents:

1. Diurnal thermoperiodicity.
2. Seasonal periodicity.
3. Homeostasis.
4. Thermoregulation.

5. Assertion: Low and high temperature causes stress in organisms.

Reasons: Organisms show specific adaptations to overcome stressful condition.

1. Both assertion and reason are true and reason is the correct explanation of assertion
2. Both assertion and reason are true but reason is not the correct explanation of assertion.
3. Assertion is true but reason is false.
4. Both assertion and reason are false.

Distinguish between : Vital Capacity of Lung and Total Lung Capacity.