Hence only Alanine can be used to determine percentage of nitrogen.
$\underset{Alanine}{\mathop{\begin{matrix}
{{H}_{2}}N-CH-COOH \\
|\,\,\,\,\,\,\,\,\, \\
\,\,\,\,\,\,\,\,\,CH{{(C{{H}_{3}})}_{2}} \\
\end{matrix}}}\,$
(પરમાણ્વીય દળ $Ag =108; Br = 80$)
$A$ નું ગણતરી કરેલ $R_f$ મૂલ્ય .......... $\times 10^{-1}$ છે.